LAB Publications in 2012-13

A 22-nt artificial microRNA mediates widespread RNA silencing in Arabidopsis. McHale, M., Eamens, A.L., Finnegan, E.J., and Waterhouse, P.M. The Plant Journal 76, 519-529 (2013).
De novo transcriptome sequence assembly and analysis of RNA silencing genes of Nicotiana benthamiana. Nakasugi, K., Crowhurst, R.N., Bally, J., Wood, C.C., Hellens, R.P., and Waterhouse, P.M. PLoS One 8, e59534.(2013).
Description of plant tRNA-derived RNA fragments (tRFs) associated with argonaute and identification of their putative targets. Loss-Morais, G., Waterhouse, P.M., and Margis, R. Biol Direct 8, 6.(2013)
C. elegans RNA-dependent RNA polymerases rrf-1 and ego-1 silence Drosophila transgenes by differing mechanisms. Duan, G., Saint, R.B., Helliwell, C.A., Behm, C.A., Wang, M.B., Waterhouse, P.M., and Gordon, K.H. Cell Mol Life Sci 70, 1469-1481.(2013).
Facile mutant identification via a single parental backcross method and application of whole genome sequencing based mapping pipelines. Allen, R.S., Nakasugi, K., Doran, R.L., Millar, A.A., and Waterhouse, P.M. Frontiers in Plant Science 4, 362. (2013).
Small RNA sequencing of Potato leafroll virus-infected plants reveals an additional subgenomic RNA encoding a sequence-specific RNA-binding protein. Hwang, Y.T., Kalischuk, M., Fusaro, A.F., Waterhouse, P.M., and Kawchuk, L. Virology 438, 61-69.(2013).
Complete genomic sequence of a Rubus yellow net virus isolate and detection of genome-wide pararetrovirus-derived small RNAs. Kalischuk, M.L., Fusaro, A.F., Waterhouse, P.M., Pappu, H.R., and Kawchuk, L.M. Virus Res 178, 306-313. (2013).
Modeling antiviral resistance in plants. Anderssen, R.S., and Waterhouse, P.M. Methods Mol Biol 894, 139-154.(2012).
Isolation and analysis of small RNAs from virus-infected plants. Curtin, S.J., Zsogon, A., Watson, J.M., and Waterhouse, P.M. Methods Mol Biol 894, 173-189.(2012).
DRB2 is required for microRNA biogenesis in Arabidopsis thaliana. Eamens, A.L., Kim, K.W., Curtin, S.J., and Waterhouse, P.M. DRB2, DRB3 and DRB5 function in a non-canonical microRNA pathway in Arabidopsis thaliana. PLoS One 7, e35933. (2012a).
Eamens, A.L., Wook Kim, K., and Waterhouse, P.M. Plant Signal Behav 7, 1224-1229.(2012).
The Enamovirus P0 protein is a silencing suppressor which inhibits local and systemic RNA silencing through AGO1 degradation. Fusaro, A.F., Correa, R.L., Nakasugi, K., Jackson, C., Kawchuk, L., Vaslin, M.F., and Waterhouse, P.M. Virology 426, 178-187.(2012).
Advanced engineering of lipid metabolism in Nicotiana benthamiana using a draft genome and the V2 viral silencing-suppressor protein. Naim, F., Nakasugi, K., Crowhurst, R.N., Hilario, E., Zwart, A.B., Hellens, R.P., Taylor, J.M., Waterhouse, P.M., and Wood, C.C. PLoS One 7, e52717.(2012).
Gene silencing in Arabidopsis spreads from the root to the shoot, through a gating barrier, by template-dependent, nonvascular, cell-to-cell movement. Liang, D., White, R.G., and Waterhouse, P.M. Plant Physiology 159, 984-1000.(2012).